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Study on diversity of endophytic bacterial communities in seeds of hybrid maize and their parental lines

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Abstract

The seeds of plants are carriers of a variety of beneficial bacteria and pathogens. Using the non-culture methods of building 16S rDNA libraries, we investigated the endophytic bacterial communities of seeds of four hybrid maize offspring and their respective parents. The results of this study show that the hybrid offspring Yuyu 23, Zhengdan958, Jingdan 28 and Jingyu 11 had 3, 33, 38 and 2 OTUs of bacteria, respectively. The parents Ye 478, Chang 7-2, Zheng 58, Jing 24 and Jing 89 had 12, 36, 6, 12 and 2 OTUs, respectively. In the hybrid Yuyu 23, the dominant bacterium Pantoea (73.38 %) was detected in its female parent Ye 478, and the second dominant bacterium of Sphingomonas (26.62 %) was detected in both its female (Ye 478) and male (Chang 7-2) parent. In the hybrid Zhengdan 958, the first dominant bacterium Stenotrophomonas (41.67 %) was detected in both the female (Zheng 58) and male (Chang 7-2) parent. The second dominant bacterium Acinetobacter (9.26 %) was also the second dominant bacterium of its male parent. In the hybrid Jingdan 28, the second dominant bacterium Pseudomonas (12.78 %) was also the second dominant bacterium of its female parent, and its third dominant bacterium Sphingomonas (9.90 %) was the second dominant bacterium of its male parent and detected in its female parent. In the hybrid Jingyu 11, the first dominant bacterium Leclercia (73.85 %) was the third dominant bacterium of its male parent, and the second dominant bacterium Enterobacter (26.15 %) was detected in its male parent. As far as we know, this was the first research reported in China on the diversity of the endophytic bacterial communities of the seeds of various maize hybrids with different genotypes.

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Abbreviations

CTAB:

Cetyltrimethylammonium bromide

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 30770069), the Science Foundation of Beijing (No. 5092004). National Science and Technology Support Plans of China (2012BAK17B11) and International Science and Technology Cooperation Projects of Beijing (Z111105054611011). We would like to thank Professor Jiuran Zhao and Fengge Wang at Beijing Academy of Agriculture and Forestry Sciences for their assistance with supplying the seed sample of maize. We would also like to thank Christine Verhille at the University of British Columbia for her assistance with English language and grammatical editing of the manuscript.

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Authors and Affiliations

  1. College of Life Sciences, Capital Normal University, Beijing, 100048, People’s Republic of China

    Yang Liu, Shan Zuo, Yuanyuan Zou & Wei Song

  2. Maize Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, People’s Republic of China

    Liwen Xu

  3. China National Research Institute of Food and Fermentation Industries, Beijing, 100027, People’s Republic of China

    Yang Liu

  4. China Center of Industrial Culture Collection, China National Research Institute of Food and Fermentation Industries, Beijing, 100027, People’s Republic of China

    Yang Liu

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  1. Yang Liu
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  2. Shan Zuo
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  3. Liwen Xu
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  4. Yuanyuan Zou
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  5. Wei Song
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Correspondence to Wei Song.

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Communicated by Ursula Priefer.

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Liu, Y., Zuo, S., Xu, L. et al. Study on diversity of endophytic bacterial communities in seeds of hybrid maize and their parental lines. Arch Microbiol 194, 1001–1012 (2012). https://doi.org/10.1007/s00203-012-0836-8

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  • DOI: https://doi.org/10.1007/s00203-012-0836-8

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